Mechanosensitive genomic enhancers potentiate the cellular response to matrix stiffness

• 成果类型: Article
• 署名作者: Cosgrove, Brian D.; Bounds, Lexi R.; Taylor, Carson Key; Su, Alan L.; Rizzo, Anthony J.; Barrera, Alejandro; Sun, Tongyu; Safi, Alexias; Song, Lingyun; Whitlow, Thomas; Tata, Aleksandra; Iglesias, Nahid; Diao, Yarui; Tata, Purushothama Rao; Hoffman, Brenton D.; Crawford, Gregory E.; Gersbach, Charles A.
• 署名单位: Duke University; Duke University; Duke University; Duke University; Duke University; Duke University; Duke University; Duke University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8075; 1095-9203
• DOI: 10.1126/science.adl1988
• 发表日期: 2025-12-11
• 页码: eadl1988
• 关键词: extracellular-matrix; growth-factor; SUBSTRATE STIFFNESS; regulatory elements; gene; yap; transcription; mechanotransduction; expression; ANOIKIS

摘要

Epigenetic control of gene expression and cellular phenotype is influenced by changes in the local microenvironment, yet how mechanical cues precisely influence epigenetic state to regulate transcription remains largely unmapped. In this study, we combined genome-wide epigenome profiling, epigenome editing, and phenotypic and single-cell RNA sequencing CRISPR screening to identify a class of genomic enhancers that responds to the mechanical microenvironment. These mechanoenhancers can be preferentially activated on either soft or stiff extracellular matrix contexts and regulate transcription to influence critical cell functions including apoptosis, adhesion, proliferation, and migration. Epigenetic editing of mechanoenhancers reprograms the cellular response to the mechanical microenvironment and modulates the activation of disease-related genes in lung fibroblasts from healthy and fibrotic donors. Epigenetic editing of mechanoenhancers holds potential for precise targeting of mechanically driven diseases.