Mucosal vaccination in mice provides protection from diverse respiratory threats

• 成果类型: Article
• 署名作者: Zhang, Haibo; Floyd, Katharine; Fang, Zhuoqing; Hoffmann, Filipe Araujo; Lee, Audrey; Froggatt, Heather Marie; Bharj, Gurpreet; Xie, Xia; Eppler, Haleigh B.; Santagata, Jordan Mariah; Wang, Yanli; Hu, Mengyun; Fox, Christopher B.; Arunachalam, Prabhu S.; Baric, Ralph; Suthar, Mehul S.; Pulendran, Bali
• 署名单位: Stanford Medicine; Stanford University; Emory University; Children's Healthcare of Atlanta (CHOA); University of North Carolina; University of North Carolina Chapel Hill; University of North Carolina School of Medicine; University of Washington; University of Washington Seattle; University of Arizona; University of Arizona Health Sciences; Stanford University; Stanford Medicine; Stanford Medicine; Stanford University; Agency for Science Technology & Research (A*STAR); A*STAR - Genome Institute of Singapore (GIS); Duke University; Utah System of Higher Education; Utah State University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-8075; 1095-9203
• DOI: 10.1126/science.aea1260
• 发表日期: 2026-05-21
• 页码: eaea1260
• 关键词: BCG VACCINATION; mortality; vaccines; receptor; cells; CORONAVIRUSES; infection; diseases; birth; lung

摘要

Traditional vaccines target specific pathogens, limiting their scope against diverse respiratory threats. We describe an intranasal liposomal formulation combining toll- like receptor 4 and 7/8 ligands with a model antigen, ovalbumin, which provided broad, durable protection in mice for at least 3 months against infection with severe acute respiratory syndrome coronavirus 2 (SaRS- CoV- 2) and Staphylococcus aureus. In addition, the vaccine protected mice from other viruses (SaRS- CoV- 2, SaRS, SHC014 coronavirus), bacteria (Acinetobacter baumannii), and allergens. Protection was mediated by persistent ovalbumin- specific CD4(+ )and CD8(+ )memory T cells that imprinted alveolar macrophages (aMs), enhancing antigen presentation and antiviral immunity. Following infection, vaccinated mice mounted rapid pathogen- specific T cell and antibody responses and formed ectopic lymphoid structures in the lung. These results reveal a class of universal vaccines against diverse respiratory threats.