Cell division machinery drives cell- specific gene activation during differentiation in Bacillus subtilis

• 成果类型: Article
• 署名作者: Chareyre, Sylvia; Li, Xuesong; Anjuwon, Brandon R.; Updegrove, Taylor B.; Clifford, Sarah; Brogan, Anna P.; Su, Yijun; Zhang, Lixia; Chen, Jiji; Shroff, Hari; Ramamurthia, Kumaran S.
• 署名单位: National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI); National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB); Howard Hughes Medical Institute; National Institutes of Health (NIH) - USA
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-15402
• DOI: 10.1073/pnas.2400584121
• 发表日期: 2024-03-26
• 关键词: transcription factor sigma(f); sporulation protein spoiie; chromosome segregation; membrane curvature; ftsz-rings; localization; site; phosphatase; expression; governs

摘要

When faced with starvation, the bacterium Bacillus subtilis transforms itself into a dormant cell type called a spore. Sporulation initiates with an asymmetric division event, which requires the relocation of the core divisome components FtsA and FtsZ, after which the sigma factor aF is exclusively activated in the smaller daughter cell. Compartment- specific activation of aF requires the SpoIIE phosphatase, which displays a biased localization on one side of the asymmetric division septum and associates with the structural protein DivIVA, but the mechanism by which this preferential localization is achieved is unclear. Here, we isolated a variant of DivIVA that indiscriminately activates aF in both daughter cells due to promiscuous localization of SpoIIE, which was corrected by overproduction of FtsA and FtsZ. We propose that the core components of the redeployed cell division machinery drive the asymmetric localization of DivIVA and SpoIIE to trigger the initiation of the sporulation program.