NRF2 is a spatiotemporal metabolic hub essential for the polyfunctionality of Th2 cells

• 成果类型: Article
• 署名作者: Choi, Garam; Ju, Hye-Yeon; Bok, Jahyun; Choi, Jungseo; Shin, Jae Woo; Oh, Hansol; Jeon, Yeojin; Kim, Jiyeon; Kim, Daehong; Moon, Heesu; Lee, Jeong-Eun; Keum, Young-Sam; Kim, You-Me; Kim, Hye Young; Park, Sung Ho; Han, Mi-Ryung; Chung, Yeonseok
• 署名单位: Seoul National University (SNU); Incheon National University; Seoul National University (SNU); Ulsan National Institute of Science & Technology (UNIST); Dongguk University; Korea Advanced Institute of Science & Technology (KAIST)
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-15368
• DOI: 10.1073/pnas.2319994121
• 发表日期: 2024-07-09
• 关键词: acute lung injury; cd4(+) t-cells; airway inflammation; nadph oxidase; ppar-gamma; innate; activation; expression; t(h)2; immunity

摘要

Upon encountering allergens, CD4 + T cells differentiate into IL- 4- producing Th2 cells in lymph nodes, which later transform into polyfunctional Th2 cells producing IL- 5 and IL- 13 in inflamed tissues. However, the precise mechanism underlying their polyfunctionality remains elusive. In this study, we elucidate the pivotal role of NRF2 in polyfunctional Th2 cells in murine models of allergic asthma and in human Th2 cells. We found that an increase in reactive oxygen species (ROS) in immune cells infiltrating the lungs is necessary for the development of eosinophilic asthma and polyfunctional Th2 cells in vivo. Deletion of the ROS sensor NRF2 specifically in T cells, but not in dendritic cells, significantly abolished eosinophilia and polyfunctional Th2 cells in the airway. Mechanistically, NRF2 intrinsic to T cells is essential for inducing optimal oxidative phosphorylation and glycolysis capacity, thereby driving Th2 cell polyfunctionality independently of IL- 33, partially by inducing PPAR gamma . Treatment with an NRF2 inhibitor leads to a substantial decrease in polyfunctional Th2 cells and subsequent eosinophilia in mice and a reduction in the production of Th2 cytokines from peripheral blood mononuclear cells in asthmatic patients. These findings highlight the critical role of Nrf2 as a spatial and temporal metabolic hub that is essential for polyfunctional Th2 cells, suggesting potential therapeutic implications for allergic diseases.