WAV E3 ubiquitin ligases mediate degradation of IAA32/34 in the TMK1-mediated auxin signaling pathway during apical hook development

• 成果类型: Article
• 署名作者: Wang, Jun-Li; Wang, Ming; Zhang, Li; Li, You-Xia; Li, Jing-Jing; Li, Yu-Yang; Pu, Zuo-Xian; Li, Dan-Yang; Liu, Xing-Nan; Guo, Wang; Di, Dong-Wei; Li, Xiao-Feng; Guo, Guang-Qin; Wu, Lei
• 署名单位: Lanzhou University; Lanzhou University; Nanjing Agricultural University; Fujian Agriculture & Forestry University; Chinese Academy of Sciences; Nanjing Institute of Soil Science, CAS
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14925
• DOI: 10.1073/pnas.2314353121
• 发表日期: 2024-04-23
• 关键词: h+-atpases; proteins; FAMILY

摘要

Auxin regulates plant growth and development through downstream signaling pathways, including the best - known SCF TIR1/AFB - Aux/IAA - ARF pathway and several other less characterized noncanonical pathways. Recently, one SCF TIR1/AFB - independent noncanonical pathway, mediated by Transmembrane Kinase 1 (TMK1), was discovered through the analyses of its functions in Arabidopsis apical hook development. Asymmetric accumulation of auxin on the concave side of the apical hook triggers DAR1 - catalyzed release of the C - terminal of TMK1, which migrates into the nucleus, where it phosphorylates and stabilizes IAA32/34 to inhibit cell elongation, which is essential for full apical hook formation. However, the molecular factors mediating IAA32/34 degradation have not been identified. Here, we show that proteins in the CYTOKININ INDUCED ROOT WAVING 1 (CKRW1)/WAVY GROWTH 3 (WAV3) subfamily act as E3 ubiquitin ligases to target IAA32/34 for ubiquitination and degradation, which is inhibited by TMK1c - mediated phosphorylation. This antagonistic interaction between TMK1c and CKRW1/WAV3 subfamily E3 ubiquitin ligases regulates IAA32/34 levels to control differential cell elongation along opposite sides of the apical hook.