Efr3b is essential for social recognition by modulating the excitability of CA2 pyramidal neurons

• 成果类型: Article
• 署名作者: Wei, Xiaojie; Wang, Jing; Yang, Enlu; Zhang, Yiping; Qian, Qi; Li, Xuekun; Huang, Fude; Sun, Binggui
• 署名单位: Zhejiang University; Zhejiang University; Zhejiang University; Zhejiang University; Zhejiang University; Chinese Academy of Medical Sciences - Peking Union Medical College; Zhejiang University; Zhejiang University; Shaoxing University; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14722
• DOI: 10.1073/pnas.2314557121
• 发表日期: 2024-01-16
• 关键词: plasma-membrane; cognitive functions; adult neurogenesis; rolling blackout; amyloid-beta; animal-model; mouse model; phosphoinositides; hyperexcitability; pi4kiii-alpha

摘要

CA2 pyramidal neurons ( PNs) are associated with social behaviors. The mechanisms, however, remain to be fully investigated. Here, we report that Efr3b, a protein essential for phospholipid metabolism at the plasma membrane, is widely expressed in the brain, especially in the hippocampal CA2/CA3 areas. To assess the functional significance of Efr3b in the brain, we generated Efr3bf/f mice and crossed them with Nestin-cre mice to delete Efr3b specifically in the brain. We find that Efr3b deficiency in the brain leads to deficits of social novelty recognition and hypoexcitability of CA2 PNs. We then knocked down the expression of Efr3b specifically in CA2 PNs of C57BL/6J mice, and our results showed that reducing Efr3b in CA2 PNs also resulted in deficits of social novelty recognition and hypoexcitability of CA2 PNs. More interestingly, restoring the expression of Efr3b in CA2 PNs enhances their excitability and improves social novelty recognition in Efr3b-deficient mice. Furthermore, direct activation of CA2 PNs with chemogenetics improves social behaviors in Efr3b-deficient mice. Together, our data suggest that Efr3b is essential for social novelty by modulating the excitability of CA2 PNs.