Extracellular vesicle formation in Euryarchaeota is driven by a small GTPase

• 成果类型: Article
• 署名作者: Mills, Joshua; Gebhard, L. Johanna; Schubotz, Florence; Shevchenko, Anna; Speth, Daan R.; Liao, Yan; Duggin, Iain G.; Marchfelder, Anita; Erdmann, Susanne
• 署名单位: Max Planck Society; University of Bremen; Max Planck Society; Max Planck Society; University of Technology Sydney; Ulm University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14706
• DOI: 10.1073/pnas.2311321121
• 发表日期: 2024-02-26
• 关键词: archaeon haloferax-volcanii; membrane-trafficking system; cell-division; complex; protein; EVOLUTION; rnas; prediction; families; strains

摘要

Since their discovery, extracellular vesicles (EVs) have changed our view on how organisms interact with their extracellular world. EVs are able to traffic a diverse array of molecules across different species and even domains, facilitating numerous functions. In this study, we investigate EV production in Euryarchaeota, using the model organism Haloferax volcanii. We uncover that EVs enclose RNA, with specific transcripts preferentially enriched, including those with regulatory potential, and conclude that EVs can act as an RNA communication system between haloarchaea. We demonstrate the key role of an EV- associated small GTPase for EV formation in H. volcanii that is also present across other diverse evolutionary branches of Archaea. We propose the name, ArvA, for the identified family of archaeal vesiculating GTPases. Additionally, we show that two genes in the same operon with arvA (arvB and arvC) are also involved in EV formation. Both, arvB and arvC, are closely associated with arvA in the majority of other archaea encoding ArvA. Our work demonstrates that small GTPases involved in membrane deformation and vesiculation, ubiquitous in Eukaryotes, are also present in Archaea and are widely distributed across diverse archaeal phyla.