Modulation of diabetes- related retinal pathophysiology by PTX3

• 成果类型: Article
• 署名作者: Pathak, Varun; Bertelli, Pietro M.; Pedrini, Edoardo; Harkin, Kevin; Peixoto, Elisa; Allen, Lynsey - Dawn; Mcloughlin, Kiran; Chavda, Natasha D.; Hamill, Kevin J.; Fuchs, Jasenka Guduric -; Inforzato, Antonio; Bottazzi, Barbara; Stitt, Alan W.; Medina, Reinhold J.
• 署名单位: Queens University Belfast; Humanitas University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14384
• DOI: 10.1073/pnas.2320034121
• 发表日期: 2024-10-08
• 关键词: long pentraxin ptx3; pattern-recognition molecule; innate immunity; tissue-repair; retinopathy; degeneration; phagocytosis; inflammation; protein; LIMITS

摘要

Diabetic retinopathy (DR) is a common complication of diabetes characterized by vascular pathology and neuroinflammation. Pentraxin 3 (PTX3) is a soluble pattern recognition molecule that functions at the crossroads between innate immunity, inflammation, and tissue remodeling. DR is known to involve inflammatory pathways, although the potential relevance of PTX3 has not been explored. We found that PTX3 protein levels increased in the retina of diabetic mice. Similarly, evaluation of a publicly available transcriptomic human dataset revealed increased PTX3 expression in DR with diabetic macular edema and proliferative retinopathy, when compared to nondiabetic retinas or diabetic retinas without complications. To further understand the role of PTX3 within DR, we employed the streptozotocin- induced diabetes model in PTX3 knockout mice (PTX3KO), which were followed up for 9 mo to evaluate hallmarks of disease progression. In diabetic PTX3KO mice, we observed decreased reactive gliosis, diminished microglia activation, and reduced vasodegeneration, when compared to diabetic PTX3 wild- type littermates. The decrease in DR-associated pathological features in PTX3KO retinas translated into preserved visual function, as evidenced by improved optokinetic response, restored b- wave amplitude in electroretinograms, and attenuated neurodegeneration. We showed that PTX3 induced an inflammatory phenotype in human retinal macroglia, characterized by GFAP upregulation and increased secretion of IL6 and PAI-1. We confirmed that PTX3 was required for TNF-alpha-induced reactive gliosis, as PTX3KO retinal explants did not up- regulate GFAP in response to TNF-alpha. This study reveals a unique role for PTX3 as an enhancer of sterile inflammation in DR, which drives pathogenesis and ultimately visual impairment.