eLemur: A cellular-resolution 3D atlas of the mouse lemur brain

• 成果类型: Article
• 署名作者: Jeon, Hyungju; Kim, Jiwon; Kim, Jayoung; Choi, Yoon Kyoung; Ho, Chun Lum Andy; Pifferi, Fabien; Huber, Daniel; Feng, Linqing; Kim, Jinhyun
• 署名单位: Korea Institute of Science & Technology (KIST); Korea University; University of Geneva; Centre National de la Recherche Scientifique (CNRS); CNRS - Institute of Ecology & Environment (INEE); Museum National d'Histoire Naturelle (MNHN); Sungkyunkwan University (SKKU); Korea Institute of Science & Technology (KIST)
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14354
• DOI: 10.1073/pnas.2413687121
• 发表日期: 2024-12-10
• 关键词: visual-cortex; primate model; neuroscience; ontology; biology; vglut2; adult

摘要

The gray mouse lemur ( Microcebus murinus), one of the smallest living primates, emerges as a promising model organism for neuroscience research. This is due to its genetic similarity to humans, its evolutionary position between rodents and humans, and its primate- like features encapsulated within a rodent- sized brain. Despite its potential, the absence of a comprehensive reference brain atlas impedes the progress of research endeavors in this species, particularly at the microscopic level. Existing references have largely been confined to the macroscopic scale, lacking detailed anatomical information. Here, we present eLemur, a unique resource, comprising a repository of high- resolution brain- wide images immunostained with multiple cell type and structural markers, elucidating the cyto- and chemoarchitecture of the mouse lemur brain. Additionally, it encompasses a segmented two- dimensional reference and 3D anatomical brain atlas delineated into cortical, subcortical, and other vital regions. Furthermore, eLemur includes a comprehensive 3D cell atlas, providing densities and spatial distributions of non- neuronal and neuronal cells across the mouse lemur brain. Accessible via a web- based viewer (https://eeum-brain.com/#/lemurdatasets), the eLemur resource streamlines data sharing and integration, fostering the exploration of different hypotheses and experimental designs using the mouse lemur as a model organism. Moreover, in conjunction with the growing 3D datasets for rodents, nonhuman primates, and humans, our eLemur 3D digital framework enhances the potential for comparative analysis and translation research, facilitating the integration of extensive rodent study data into human studies.