Cell-cell transfer of adaptation traits benefits kin and actor in a cooperative microbe

• 成果类型: Article
• 署名作者: Subedi, Kalpana; Roy, Pravas C.; Saiz, Brandon; Basile, Franco; Wall, Daniel
• 署名单位: University of Wyoming; University of Wyoming
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-14170
• DOI: 10.1073/pnas.2402559121
• 发表日期: 2024-07-23
• 关键词: outer-membrane exchange; myxococcus-xanthus; antibiotic ta; myxobacteria; RECOGNITION; EVOLUTION; motility; mutants; biogenesis; database

摘要

Microbes face many physical, chemical, and biological insults from their environments. In response, cells adapt, but whether they do so cooperatively is poorly understood. Here, we use a model social bacterium, Myxococcus xanthus, to ask whether adapted traits are transferable to na & iuml;ve kin. To do so we isolated cells adapted to detergent stresses and tested for trait transfer. In some cases, strain- mixing experiments increased sibling fitness by transferring adaptation traits. This cooperative behavior depended on a kin recognition system called outer membrane exchange (OME) because mutants defective in OME could not transfer adaptation traits. Strikingly, in mixed stressed populations, the transferred trait also benefited the adapted (actor) cells. This apparently occurred by alleviating a detergent- induced stress response in kin that otherwise killed actor cells. Additionally, this adaptation trait when transferred also conferred resistance against a lipoprotein toxin delivered to targeted kin. Based on these and other findings, we propose a model for stress adaptation and how OME in myxobacteria promotes cellular cooperation in response to environmental stresses.