The ergodicity question when imaging DNA conformation using liquid cell electron microscopy
成果类型：
Article
署名作者：
Li, Jia- Ye; Liu, Fan; Xu, Jing; Kim, Ye-Jin; Kwon, Oh-Hoon; Xia, Bin; Wang, Huan; Granick, Steve
署名单位：
Peking University; Peking University; Ulsan National Institute of Science & Technology (UNIST); Institute for Basic Science - Korea (IBS); University of Massachusetts System; University of Massachusetts Amherst
刊物名称：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN/ISSBN：
0027-13998
DOI：
10.1073/pnas.2314797121
发表日期：
2024-01-16
关键词：
摘要：
Assessing the ergodicity of graphene liquid cell electron microscope measurements, we report that loop states of circular DNA interconvert reversibly and that loop numbers fol-low the Boltzmann distribution expected for this molecule in bulk solution, provided that the electron dose is low (80- keV electron energy and electron dose rate 1-20 e(-) angstrom(-2) s(-1)). This imaging technique appears to act as a slow motion camera that reveals equilibrated distributions by imaging the time average of a few molecules without the need to image a spatial ensemble.