ZmPILS6 is an auxin efflux carrier required for maize root morphogenesis
成果类型：
Article
署名作者：
Cowling, Craig L.; Homayouni, Arielle L.; Callwood, Jodi B.; Mcreynolds, Maxwell R.; Khor, Jasper; Ke, Haiyan; Draves, Melissa A.; Dehesh, Katayoon; Walley, Justin W.; Strader, Lucia C.; Kelley, Dior R.
署名单位：
Iowa State University; Duke University; Iowa State University; University of California System; University of California Riverside
刊物名称：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN/ISSBN：
0027-13947
DOI：
10.1073/pnas.2313216121
发表日期：
2024-05-28
关键词：
arabidopsis-thaliana
branching density
gene-expression
transport
protein
pin
cell
initiation
phenotypes
FAMILY
摘要：
Plant root systems play a pivotal role in plant physiology and exhibit diverse phenotypic traits. Understanding the genetic mechanisms governing root growth and development in model plants like maize is crucial for enhancing crop resilience to drought and nutrient limitations. This study focused on identifying and characterizing ZmPILS6, an annotated auxin efflux carrier, as a key regulator of various crown root traits in maize. ZmPILS6 - modified roots displayed reduced network area and suppressed lateral root formation, which are desirable traits for the steep, cheap, and deep ideotype. The research revealed that ZmPILS6 localizes to the endoplasmic reticulum and plays a vital role in controlling the spatial distribution of indole - 3 - acetic acid (IAA or auxin) in primary roots. The study also demonstrated that ZmPILS6 can actively efflux IAA when expressed in yeast. Furthermore, the loss of ZmPILS6 resulted in significant proteome remodeling in maize roots, particularly affecting hormone signaling pathways. To identify potential interacting partners of ZmPILS6, a weighted gene coexpression analysis was performed. Altogether, this research contributes to the growing knowledge of essential genetic determinants governing maize root morphogenesis, which is crucial for guiding agricultural improvement strategies.