Circadian and environmental signal integration in a natural population of Arabidopsis

• 成果类型: Article
• 署名作者: Nishio, Haruki; Ramirez, Dora L. Cano-; Muranaka, Tomoaki; Dantas, Liiza Lane de Barros; Honjo, Mie N.; Sugisaka, Jiro; Kudoh, Hiroshi; Dodd, Antony N.
• 署名单位: Kyoto University; Shiga University; University of Cambridge; University of Bristol; Nagoya University; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC); John Innes Center
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13913
• DOI: 10.1073/pnas.2402697121
• 发表日期: 2024-08-27
• 关键词: light-responsive promoter; plastid sigma-factor; chloroplast transcription; temperature response; rna-polymerase; uv-b; clock; expression; stress; gene

摘要

Plants sense and respond to environmental cues during 24 h fluctuations in their environment. This requires the integration of internal cues such as circadian timing with environmental cues such as light and temperature to elicit cellular responses through signal transduction. However, the integration and transduction of circadian and environmental signals by plants growing in natural environments remains poorly understood. To gain insights into 24 h dynamics of environmental signaling in nature, we performed a field study of signal transduction from the nucleus to chloroplasts in a natural population of Arabidopsis halleri. Using several modeling approaches to interpret the data, we identified that the circadian clock and temperature are key regulators of this pathway under natural conditions. We identified potential time- delay steps between pathway components, and diel fluctuations in the response of the pathway to temperature cues that are reminiscent of the process of circadian gating. We found that our modeling framework can be extended to other signaling pathways that undergo diel oscillations and respond to environmental cues. This approach of combining studies of gene expression in the field with modeling allowed us to identify the dynamic integration and transduction of environmental cues, in plant cells, under naturally fluctuating diel cycles.