Architecture- driven quantitative nanoscopy maps cytoskeleton remodeling

• 成果类型: Article
• 署名作者: Liu, Wenjie; Yao, Yushi; Meng, Jia; Qian, Shuhao; Han, Yubing; Zhou, Lingxi; Wang, Tao; Chen, Youhua; Chen, Lingmei; Ye, Zitong; Xu, Liang; Zhang, Meng; Qiu, Jianrong; Han, Tao; Liu, Xu; Kuang, Cuifang; Ding, Zhihua; Liu, Zhiyi
• 署名单位: Zhejiang University; Zhejiang University; University of Oxford; Zhejiang University; Huazhong University of Science & Technology
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13658
• DOI: 10.1073/pnas.2410688121
• 发表日期: 2024-10-15
• 关键词: microtubule dynamic instability; whole cells; GROWTH; quantification; polarization; migration; LIFE

摘要

Cytoskeleton remodeling which generates force and orchestrates signaling and trafficking to govern cell migration remains poorly understood, partly due to a lack of an investigation tool with high system flexibility, spatiotemporal resolution, and computational sensitivity. Herein, we developed a multimodal superresolution imaging system-based architecture- driven quantitative (ADQ) framework in spatiotemporal- angular hyperspace to enable both identification of the optimal imaging mode with well- balanced fidelity and phototoxicity and accurate postcharacterization of microtubule remodeling. In the ADQ framework, a pixel/voxel-wise metric reflecting heterogeneous intertubule alignment was proposed with improved sensitivity over previous efforts and further incorporated with temporal features to map dynamic microtubule rearrangements. The ADQ framework was verified by assessing microtubule remodeling in drug- induced (de)polymerization, lysosome transport, and migration. Different remodeling patterns from two migration modes were successfully revealed by the ADQ framework, with a front-rear polarization for individual directed migration and a contact site-centered polarization for cell-cell interaction- induced migration in an immune response model. Meanwhile, these migration modes were found to have consistent orientation changes, which exhibited the potential of predicting migration trajectory.