Aflatoxin B1 and Epstein-Barr virus- induced CCL22 expression stimulates B cell infection

• 成果类型: Article
• 署名作者: Maroui, Mohamed Ali; Odongo, Grace Akinyi; Mundo, Lucia; Manara, Francesca; Mure, Fabrice; Fusil, Floriane; Jay, Antonin; Gheit, Tarik; Michailidis, Thanos M.; Ferrara, Domenico; Leoncini, Lorenzo; Murray, Paul; Manet, Evelyne; Ohlmann, Theophile; De Boevre, Marthe; De Saeger, Sarah; Cosset, Francois-Loic; Lazzi, Stefano; Accardi, Rosita; Herceg, Zdenko; Gruffat, Henri; Khoueiry, Rita
• 署名单位: Institut National de la Sante et de la Recherche Medicale (Inserm); Centre National de la Recherche Scientifique (CNRS); Universite Claude Bernard Lyon 1; World Health Organization; International Agency for Research on Cancer (IARC); University of Limerick; University of Limerick; University of Siena; Ghent University; University of Johannesburg
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13494
• DOI: 10.1073/pnas.2314426121
• 发表日期: 2024-04-16
• 关键词: regulatory t-cells; ccl17; immunotoxicity; lymphocytes; RECRUITMENT; activation; carcinoma; migration; lymphomas; cancer

摘要

Epstein-Barr Virus (EBV) infects more than 90% of the adult population worldwide. EBV infection is associated with Burkitt lymphoma (BL) though alone is not sufficient to induce carcinogenesis implying the involvement of co- factors. BL is endemic in African regions faced with mycotoxins exposure. Exposure to mycotoxins and oncogenic viruses has been shown to increase cancer risks partly through the deregulation of the immune response. A recent transcriptome profiling of B cells exposed to aflatoxin B1 (AFB1) revealed an upregulation of the Chemokine ligand 22 (CCL22) expression although the underlying mechanisms were not investigated. Here, we tested whether mycotoxins and EBV exposure may together contribute to endemic BL (eBL) carcinogenesis via immunomodulatory mechanisms involving CCL22. Our results revealed that B cells exposure to AFB1 and EBV synergistically stimulated CCL22 secretion via the activation of Nuclear Factor - kappa B pathway. By expressing EBV latent genes in B cells, we revealed that elevated levels of CCL22 result not only from the expression of the latent membrane protein LMP1 as previously reported but also from the expression of other viral latent genes. Importantly, CCL22 overexpression resulting from AFB1- exposure in vitro increased EBV infection through the activation of phosphoinositide-3- kinase pathway. Moreover, inhibiting CCL22 in vitro and in humanized mice in vivo limited EBV infection and decreased viral genes expression, supporting the notion that CCL22 overexpression plays an important role in B cell infection. These findings unravel new mechanisms that may underpin eBL development and identify novel pathways that can be targeted in drug development.