SARS- CoV-2 spike does not interact with the T cell receptor or directly activate T cells

• 成果类型: Article
• 署名作者: Gaglione, Stephanie A.; Rosales, Tatiana J.; Schmidt-Hong, Laura; Baker, Brian M.; Birnbaum, Michael E.
• 署名单位: Massachusetts Institute of Technology (MIT); Massachusetts Institute of Technology (MIT); University of Notre Dame; University of Notre Dame; Massachusetts Institute of Technology (MIT); Harvard University; Massachusetts Institute of Technology (MIT); Ragon Institute
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13451
• DOI: 10.1073/pnas.2406615121
• 发表日期: 2024-07-30
• 关键词: recognition; antibody; tcr

摘要

dren, which resembles superantigen- induced toxic shock syndrome. Recent work has suggested that the SARS- CoV- 2 spike (S) protein could act as a superantigen by binding T cell receptors (TCRs) and inducing broad antigen- independent T cell sites near the S receptor- binding domain, in addition to a site with homology to known neurotoxins. We experimentally examined the mechanism underpinning this resonance of recombinantly expressed S protein and TCR revealed no detectable wild- type and prefusion- stabilized forms, demonstrated their functionality in a cell line assay, and observed no transduction, activation, or stimulation of proliferation of CD8(+ )T cells. We conclude that it is unlikely that the SARS- CoV- 2 spike protein engages nonspecifically with TCRs or has superantigenic character.