Alveolar macrophage function is impaired following inhalation of berry e- cigarette vapor

• 成果类型: Article
• 署名作者: Kulle, Amelia; Li, Ziyi; Kwak, Ashley; Mancini, Mathieu; Young, Daniel; Avizonis, Daina Zofija; Groleau, Marc; Baglole, Carolyn J.; Behr, Marcel A.; King, Irah L.; Divangahi, Maziar; Langlais, David; Wang, Jing; Blagih, Julianna; Penz, Erika; Dufour, Antoine; Thanabalasuriar, Ajitha
• 署名单位: McGill University; McGill University; University of Calgary; McGill University; McGill University; McGill University; Shanghai Jiao Tong University; Chinese Academy of Sciences; Universite de Montreal; University of Saskatchewan
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13431
• DOI: 10.1073/pnas.2406294121
• 发表日期: 2024-10-01
• 关键词: neutrophil; inhibitor; migration; adhesion; cdc42; smoke

摘要

In the lower respiratory tract, the alveolar spaces are divided from the bloodstream and the external environment by only a few microns of interstitial tissue. Alveolar macrophages (AMs) defend this delicate mucosal surface from invading infections by regularly patrolling the site. AMs have three behavior modalities to achieve this goal: extending cell protrusions to probe and sample surrounding areas, squeezing the whole cell body between alveoli, and patrolling by moving the cell body around each alveolus. In this study, we found Rho GTPase, cell division control protein 42 (CDC42) expression significantly decreased after berry- flavored e- cigarette (e-cig) exposure. This shifted AM behavior from squeezing to probing. Changes in AM behavior led to a reduction in the clearance of inhaled bacteria, Pseudomonas aeruginosa. These findings shed light on pathways involved in AM migration and highlight the harmful impact of e-cig vaping on AM function.