The periaxonal space as a conduit for cerebrospinal fluid flow to peripheral organs

• 成果类型: Article
• 署名作者: Li, Xinyu; Wang, Siman; Zhang, Dianjun; Feng, Yuliang; Liu, Yingyu; Yu, Weiyang; Cui, Lulu; Harkany, Tibor; Verkhratsky, Alexei; Xia, Maosheng; Li, Baoman
• 署名单位: China Medical University; China Medical University; China Medical University; Medical University of Vienna; Karolinska Institutet; University of Manchester; University of Basque Country; State Research Institute Centre for Innovative Medicine; China Medical University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13201
• DOI: 10.1073/pnas.2400024121
• 发表日期: 2024-11-05
• 关键词: glycogen-metabolism; primary cultures; ependymal cells; f-actin; rat; SYSTEM; ca2+; fluoxetine; reveals; neurons

摘要

Mechanisms controlling the movement of the cerebrospinal fluid (CSF) toward peripheral nerves are poorly characterized. We found that, in addition to the foramina Magendie outflow could also occur along periaxonal spaces (termed PAS pathway) from the spinal cord to peripheral organs, such as the liver and pancreas. When interrogating the latter route, we found that serotonin, acting through 5- HT2B receptors expressed erization of F- actin, a cytoskeletal protein, to reduce the volume of ependymal cells. This paralleled an increased rate of PAS- mediated CSF redistribution toward peripheral organs. In the liver, CSF was received by hepatic stellate cells. CSF efflux toward peripheral organs through the PAS pathway represents a mechanism dynamically connecting the nervous system with the periphery. Our findings are compatible with the traditional theory of CSF efflux into the glymphatic system to clear metabolic waste from the cerebral parenchyma. Thus, we extend the knowledge of CSF flow and expand the understanding of connectivity between the CNS and peripheral organs.