Genetic evidence against involvement of TRPC proteins in SOCE, ROCE, and CRAC channel function

• 成果类型: Article
• 署名作者: Susperreguy, Sebastian; Yamashita, Megumi; Choi, Chan-il; Liao, Yanhong; Burch, Lauranell H.; Blankenship, Terry L.; Hayes, Erika; Sliwa, Thomas; Zhang, Yingpei; Grenet, Dagoberto; Walker, Mitzie; Plummer, Nicholas W.; Abramowitz, Joel; Kinet, Jean Pierre; Formoso, Karina; Johnson, Brandon E.; Fleig, Andrea; Hazlehurst, Lori; Penner, Reinhold; Freichel, Marc; Flockerzi, Veit; Prakriya, Murali; Birnbaumer, Lutz
• 署名单位: Pontificia Universidad Catolica Argentina; Northwestern University; Feinberg School of Medicine; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS); Huazhong University of Science & Technology; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS); National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS); National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS); Harvard University; Harvard University Medical Affiliates; Beth Israel Deaconess Medical Center; Harvard University; Harvard Medical School; The Queen's Medical Center; University of Hawaii System; Cancer Research Center of Hawaii; University of Hawaii System; West Virginia University; Saarland University; Ruprecht Karls University Heidelberg
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13193
• DOI: 10.1073/pnas.2411389121
• 发表日期: 2024-12-03
• 关键词: store; activation; cells; mice; orai; expression; knockout; subunit; lacking; stim1

摘要

Using genetically engineered mice and cell lines derived from genetically engineered mice depend obligatorily on functionally intact TRPC molecules, as SOCE monitored with have been structurally and functionally inactivated. Unexpectedly as well, we found that unaffected by ablation of all seven TRPC genes. We refer to mice and cells carrying the seven- fold disruption of TRPC genes as TRPC heptaKO mice and cells. TRPC heptaKO mice are fertile allowing the creation of a new homozygous inbred strain.