Hyperosmolarity- induced suppression of group B1 Raf-like protein kinases modulates droughtgrowth trade- off in Arabidopsis

• 成果类型: Article
• 署名作者: Kamiyama, Yoshiaki; Katagiri, Sotaro; Li, Yangdan; Yamashita, Kota; Takase, Hinano; Umezawa, Taishi
• 署名单位: Tokyo University of Agriculture & Technology; Tokyo University of Agriculture & Technology
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13184
• DOI: 10.1073/pnas.2419204121
• 发表日期: 2024-12-24
• 关键词: osmotic-stress; phosphatase 2a; abscisic-acid; GROWTH; digestion; responses; vectors; FAMILY

摘要

When plants are exposed to drought stress, there is a trade- off between plant growth and stress responses. Here, we identified a signaling mechanism for the initial steps of the drought- growth trade- off. Phosphoproteomic profiling revealed that Raf13, a B1 subgroup Raf-like kinase, is dephosphorylated under drought conditions. Raf13 and the related B1-Raf Raf15 are required for growth rather than the acquisition of osmotolerance. We also found that Raf13 interacts with B55- family regulatory subunits of protein phosphatase 2A (PP2A), which mediates hyperosmolarity- induced dephosphorylation of Raf13. In addition, Raf13 interacts with an AGC kinase INCOMPLETE ROOT HAIR ELONGATION HOMOLOG 1 (IREH1), and Raf13 and IREH1 have similar functions in regulating cellular responses that promote plant growth. Overall, our results support a model in which Raf13-IREH1 activity promotes growth under nonstressed conditions, whereas PP2A activity suppresses Raf13-IREH1 during osmotic stress to modulate the physiological trade- off between plant growth and stress responses.