Drosophila neuronal Glucose-6-Phosphatase is a modulator of neuropeptide release that regulates muscle glycogen stores via FMRFamide signaling

• 成果类型: Article
• 署名作者: Miyamoto, Tetsuya; Hedjazi, Sheida; Miyamoto, Chika; Amrein, Hubert
• 署名单位: Texas A&M University System; Texas A&M University College Station
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-13029
• DOI: 10.1073/pnas.2319958121
• 发表日期: 2024-07-23
• 关键词: transcriptional regulation; gene; expression; sleep; mutations; nutrient; BEHAVIOR; rhythms; protein; cells

摘要

Neuropeptides (NPs) and their cognate receptors are critical effectors of diverse physiological processes and behaviors. We recently reported of a noncanonical function of the Drosophila Glucose- 6- Phosphatase (G6P) gene in a subset of neurosecretory cells in the central nervous system that governs systemic glucose homeostasis in food- deprived flies. Here, we show that G6P- expressing neurons define six groups of NP- secreting cells, four in the brain and two in the thoracic ganglion. Using the glucose homeostasis phenotype as a screening tool, we find that neurons located in the thoracic ganglion expressing glucose homeostasis in starved flies. We further show that G6P is essential in FMRFaG6P neurons for attaining a prominent Golgi apparatus and secreting NPs efficiently. Finally, we establish that G6P- dependent FMRFa signaling is essential for the build- up of glycogen stores in the jump muscle which expresses the receptor for FMRFamides. We propose a general model in which the main role of G6P is to counteract glycolysis in peptidergic neurons for the purpose of optimizing the intracellular environment best suited for the expansion of the Golgi apparatus, boosting release of NPs and enhancing signaling to respective target tissues expressing cognate receptors.