The reconstruction of evolutionary dynamics of processed pseudogenes indicates deep silencing of retrobiome in naked mole rat

• 成果类型: Article
• 署名作者: Kogan, Valeria; Molodtsov, Ivan; Fleyshman, Daria I.; V. Leontieva, Olga; Koman, Igor E.; V. Gudkov, Andrei
• 署名单位: Ariel University; Roswell Park Comprehensive Cancer Center
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-12997
• DOI: 10.1073/pnas.2313581121
• 发表日期: 2024-11-05
• 关键词: transposable elements; epigenetic control; dna methylation; retrotransposon; sines; insights; repeats; identification; annotation; cancer

摘要

Approximately half of mammalian genomes are occupied by retrotransposons, highly repetitive interspersed genetic elements expanded through the mechanism of reverse transcription. The evolution of this retrobiome involved a series of explosive amplifications, presumably associated with high mutation rates, interspersed with periods of silencing. A by- product of retrotransposon activity is the formation of processed pseuWe examined the proportion of PPGs with varying degrees of deviation from their ancestor mRNAs as an indicator of the intensity of retrotranspositions at different times in the past. Our analysis revealed a high proportion of young (recently acquired) PPGs in the DNA of mice and rats, indicating significant retrobiome activity during the recent evolution of these species. The ongoing process of new PPG entries in mouse germ line DNA was confirmed by identifying diversity in PPG content within the single strain of mice, substantial deviation from their mRNAs, with a near- complete lack of PPGs without mutations, indicative of the silencing of the retrobiome in the most recent evolutionary resistance to cancer, may reflect the role played by the retrobiome in aging and cancer.