Samd7 represses short- wavelength cone genes to preserve long- wavelength cone and rod photoreceptor identity

• 成果类型: Article
• 署名作者: Volkov, Leo I.; Ogawa, Yohey; Somjee, Ramiz; Vedder, Hannah E.; Powell, Hannah E.; Poria, Deepak; Meiselman, Sam; Kefalov, Vladimir J.; Corbo, Joseph C.
• 署名单位: Washington University (WUSTL); University of California System; University of California Irvine
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-12761
• DOI: 10.1073/pnas.2402121121
• 发表日期: 2024-11-19
• 关键词: opsin expression; hormone-receptor; zebrafish; beta; transcription; regeneration; vision

摘要

The role of transcription factors in photoreceptor gene regulation is fairly well understood, but knowledge of the cell- type- specific function of transcriptional cofactors remains incomplete. Here, we show that the transcriptional corepressor samd7 promotes rod differentiation and represses short- wavelength cone genes in long- wavelength cones in zebrafish. In samd7-/- retinas, red cones are transformed into hybrid red/ultraviolet (UV) cones, green cones are absent, the number of blue cones is approximately doubled, and the number of rods is greatly reduced. We also find that mouse Samd7 represses S-opsin expression in dorsal M- cones-analogous to its role in repressing UV cone genes in zebrafish red cones. Thus, samd7 plays a key role in ensuring appropriate patterns of gene expression in rods and cone subtypes of both zebrafish and mice.