A frameshift mutation in JAZ10 resolves the growth versus defense dilemma in rice

• 成果类型: Article
• 署名作者: Li, Lei-Lei; Xiao, Yujie; Wang, Baohui; Zhuang, Yunqi; Chen, Yumeng; Lu, Jing; Lou, Yonggen; Li, Ran
• 署名单位: Zhejiang University; Zhejiang Chinese Medical University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-12751
• DOI: 10.1073/pnas.2413564121
• 发表日期: 2024-12-24
• 关键词: slender rice1; jasmonate; PATHWAY

摘要

CRISPR- Cas9 genome editing systems have revolutionized plant gene functional studies by enabling the targeted introduction of insertion- deletions (INDELs) via the nonhomologous end- joining (NHEJ) pathway. Frameshift- inducing INDELs can introduce a premature termination codon and, in other instances, can lead to the appearance of new proteins. Here, we found that mutations in the rice jasmonate (JA) signaling gene OsJAZ10 by CRISPR- Cas9- based genome editing did not affect canonical JA signaling. However, a type of mutant with an INDEL that yielded a novel frameshift protein named FJ10 (Frameshift mutation of JAZ10), exhibited enhanced rice growth and increased actions disrupt the function of OsSLR1 in suppressing gibberellin- mediated growth and the function of OsFBK16 in repressing lignin- mediated defense responses, respectively. Field experiments with FJ10- expressing plants demonstrate that this protein uncouples the growth-defense tradeoff, opening broad avenues to obtain cultivars with enhanced yield without compromised defenses.