RN7SL1 may be translated under oncogenic conditions

• 成果类型: Article
• 署名作者: Hara, Tomoaki; Meng, Sikun; Tsuji, Yoshiko; Arao, Yasuko; Saito, Yoshiko; Sato, Hiromichi; Motooka, Daisuke; Uchida, Shizuka; Ishii, Hideshi
• 署名单位: University of Osaka; University of Osaka; University of Osaka; Aalborg University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-12130
• DOI: 10.1073/pnas.2312322121
• 发表日期: 2024-03-19
• 关键词: structural basis; transcription

摘要

RN7SL1 (RNA component of signal recognition particle 7SL1), a component of the signal recognition particle, is a non- coding RNA possessing a small ORF (smORF). However, whether it is translated into peptides is unknown. Here, we generated the RN7SL1-Green Fluorescent Protein (GFP) gene, in which the smORF of RN7SL1 was replaced by GFP, introduced it into 293T cells, and observed cells emitting GFP fluorescence. Furthermore, RNA-seq of GFP- positive cells revealed that they were in an oncogenic state, suggesting that RN7SL1 smORF may be translated under special conditions.