'The structure of B-ARR reveals the molecular basis of transcriptional activation by cytokinin

• 成果类型: Article
• 署名作者: Zhou, Chuan-Miao; Li, Jian-Xu; Zhang, Tian-Qi; Xu, Zhou-Geng; Ma, Miao-Lian; Zhang, Peng; Wang, Jia-Wei
• 署名单位: Chinese Academy of Sciences; Center for Excellence in Molecular Plant Sciences, CAS; Chinese Academy of Sciences; Shanghai Chenshan Botanical Garden; Chinese Academy of Sciences; ShanghaiTech University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11915
• DOI: 10.1073/pnas.2319335121
• 发表日期: 2024-01-16
• 关键词: histidine phosphotransfer proteins; phosphorelay signal-transduction; response regulators; receiver domain; arabidopsis; kinase; receptor; binding; auxin; genes

摘要

The phytohormone cytokinin has various roles in plant development, including meristem maintenance, vascular differentiation, leaf senescence, and regeneration. Prior investiga- tions have revealed that cytokinin acts via a phosphorelay similar to the two-component system by which bacteria sense and respond to external stimuli. The eventual tar- gets of this phosphorelay are type-B ARABIDOPSIS RESPONSE REGULATORS (B-ARRs), containing the conserved N-terminal receiver domain (RD), middle DNA binding domain (DBD), and C-terminal transactivation domain. While it has been established for two decades that the phosphoryl transfer from a specific histidyl residue in ARABIDOPSIS HIS PHOSPHOTRANSFER PROTEINS (AHPs) to an aspartyl residue in the RD of B-ARRS results in a rapid transcriptional response to cytokinin, the underlying molecular basis remains unclear. In this work, we determine the crystal structures of the RD-DBD of ARR1 (ARRIRD-DBD) as well as the ARRIDE DBD_DNA complex from Arabidopsis. Analyses of the ARRI DBD -DNA complex have revealed the structural basis for sequence-specific recognition of the GAT trinucleotide by ARRI. In particular, comparing the ARRIRD-DBD and ARR1DBD-DNA structures reveals that unphosphorylated ARRIRD-DBD exists in a closed conformation with extensive contacts between the RD and DBD. In vitro and vivo functional assays have further suggested that phosphorylation of the RD weakens its interaction with DBD, subsequently per- mits the DNA binding capacity of DBD, and promotes the transcriptional activity of ARR1. Our findings thus provide mechanistic insights into phosphorelay activation of gene transcription in response to cytokinin.