The zonula adherens matura redefines the apical junction of intestinal epithelia

• 成果类型: Article
• 署名作者: Mangeol, Pierre; Harroche, Dominique Massey-; Sebbagh, Michael; Richard, Fabrice; Le Bivic, Andre; Lenne, Pierre- Francois
• 署名单位: Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); Aix-Marseille Universite; Institut National de la Sante et de la Recherche Medicale (Inserm); Aix-Marseille Universite; Aix-Marseille Universite; Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB)
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11897
• DOI: 10.1073/pnas.2316722121
• 发表日期: 2024-02-27
• 关键词: adhesion molecule uvomorulin; cell-cell junctions; alpha-catenin; e-cadherin; intercellular-junctions; tight junctions; binding protein; peripheral component; cytoplasmic domain; fine-structure

摘要

Cell-cell apical junctions of epithelia consist of multiprotein complexes that organize as belts regulating cell-cell adhesion, permeability, and mechanical tension: the tight junction (zonula occludens), the zonula adherens (ZA), and the macula adherens. The prevailing dogma is that at the ZA, E- cadherin and catenins are lined with F - actin bundles that support and transmit mechanical tension between cells. Using super- resolution microscopy on human intestinal biopsies and Caco-2 cells, we show that two distinct multiprotein belts are basal of the tight junctions as the intestinal epithelia mature. The most apical is populated with nectins/afadin and lined with F- actin; the second is populated with E- cad/catenins. We name this dual - belt architecture the zonula adherens matura. We find that the apical contraction apparatus and the dual - belt organization rely on afadin expression. Our study provides a revised description of epithelial cell-cell junctions and identifies a module regulating the mechanics of epithelia.