ADPact: A versatile sensor for ADP-F-actin

• 成果类型: Article
• 署名作者: Ma, Qianqian; Han, Xiao; Zhu, Kexin; He, Danxia; Ng, Cai Xin; Miao, Yansong
• 署名单位: Nanyang Technological University; Nanyang Technological University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11849
• DOI: 10.1073/pnas.2420467122
• 发表日期: 2024-07-22
• 关键词: polymerization; mechanism; DYNAMICS; lifeact; jasplakinolide; degradation; adf/cofilin; nucleation; visualize; filaments

摘要

The dynamic organization of the actin cytoskeleton, crucial for numerous cellular processes, is intricately regulated by the nucleotide state of actin filaments (F-actin). Visualization tools for specifically detecting ADP-bound F-actin, however, remain limited. Here, we introduce ADPact, a 20-amino-acid peptide that selectively binds to ADP-F-actin. ADPact allows for the precise visualization of ADP-F-actin structures both in vitro and in eukaryotic cells, particularly under energy stress conditions where ADP-/ATP-actin ratio increases. Importantly, ADPact and its fluorescent fusion variant, ADPact-GFP, do not disrupt actin dynamics, ensuring accurate monitoring of cytoskeletal reorganization. Our findings provide a powerful tool for studying actin dynamics and offer fresh insights into the regulation of the cytoskeleton under physiological and pathological conditions.