Rab10-CAV 1 mediated intraluminal vesicle transport to migrasomes

• 成果类型: Article
• 署名作者: Li, Yong; Wen, Yiling; Li, Ying; Tan, Xinyi; Gao, Shuaixin; Fan, Peiyao; Tian, Wenmin; Wong, Catherine C. L.; Chen, Yang
• 署名单位: Peking University; Peking University; Tsinghua University; Chinese Academy of Sciences; Institute of Biophysics, CAS; University System of Ohio; Ohio State University; James Cancer Hospital & Solove Research Institute; University System of Ohio; Ohio State University; Chinese Academy of Medical Sciences - Peking Union Medical College; Peking Union Medical College
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11846
• DOI: 10.1073/pnas.2319267121
• 发表日期: 2024-07-23
• 关键词: morphogenesis; protein; rilpl2; lrrk2

摘要

Migrasomes, vesicular organelles generated on the retraction fibers of migrating cells, play a crucial role in migracytosis, mediating intercellular communication. The cargoes determine the functional specificity of migrasomes. Migrasomes harbor numerous intraluminal vesicles, a pivotal component of their cargoes. The mechanism underlying the transportation of these intraluminal vesicles to the migrasomes remains enigmatic. In this study, we identified that Rab10 and Caveolin- 1 (CAV1) mark the intraluminal vesicles in migrasomes. Transport of Rab10- CAV1 vesicles to migrasomes required the motor protein Myosin Va and adaptor proteins RILPL2. Notably, the phosphorylation of Rab10 by the kinase LRRK2 regulated this process. Moreover, CSF- 1 can be transported to migrasomes through this mechanism, subsequently fostering monocyte-macrophage differentiation in skin wound healing, which served as a proof of the physiological importance of this transporting mechanism.