Prevotella are major contributors of sialidases in the human vaginal microbiome

• 成果类型: Article
• 署名作者: Pelayo, Paula; Hussain, Fatima A.; Werlang, Caroline A.; Wu, Chloe M.; Woolston, Benjamin M.; Xiang, Claire M.; Rutt, Lindsay; France, Michael T.; Ravel, Jacques; Ribbeck, Katharina; Kwon, Douglas S.; Balskus, Emily P.
• 署名单位: Harvard University; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; Massachusetts Institute of Technology (MIT); Ragon Institute; Massachusetts Institute of Technology (MIT); Northeastern University; University System of Maryland; University of Maryland Baltimore; Harvard University; Howard Hughes Medical Institute
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11838
• DOI: 10.1073/pnas.2400341121
• 发表日期: 2024-09-03
• 关键词: bacterial; iga; neuraminidases; sialoglycans; specificity; mucins; mucus

摘要

Elevated bacterial sialidase activity in the female genital tract is strongly associated with poor health outcomes including preterm birth and bacterial vaginosis (BV). These negative effects may arise from sialidase- mediated degradation of the protective mucus layer in the cervicovaginal environment. Prior biochemical studies of vaginal bacterial sialidases have focused solely on the BV- associated organism Gardnerella vaginalis. Despite their implications for sexual and reproductive health, sialidases from other vaginal bacteria have not been characterized. Here, we show that vaginal Prevotella species produce sialidases that possess variable activity toward mucin substrates. The sequences of sialidase genes and their presence are largely conserved across clades of Prevotella from different geographies, hinting at their importance globally. Finally, we find that vaginal genomes and transcriptomes. Together, our results identify Prevotella as a critical source of sialidases in the vaginal microbiome, improving our understanding of this detrimental bacterial activity.