Halofilins as emerging bactofilin families of archaeal cell shape plasticity orchestrators

• 成果类型: Article
• 署名作者: Curtis, Zachary; Escudeiro, Pedro; Mallon, John; Leland, Olivia; Rados, Theopi; Dodge, Ashley; Andre, Katherine; Kwak, Jasmin; Yun, Kun; Isaac, Berith; Pastor, Mar Martinez; Schmid, Amy K.; Pohlschroder, Mechthild; Alva, Vikram; Bisson, Alex
• 署名单位: Brandeis University; University of Pennsylvania; Duke University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11820
• DOI: 10.1073/pnas.2401583121
• 发表日期: 2024-10-01
• 关键词: division; cytoskeleton; attachment; proteins

摘要

Bactofilins are rigid, nonpolar bacterial cytoskeletal filaments that link cellular processes to specific curvatures of the cytoplasmic membrane. Although homologs of bactofilins have been identified in archaea and eukaryotes, functional studies have remained confined to bacterial systems. Here, we characterize representatives of two families of archaeal bactofilins from the pleomorphic archaeon Haloferax volcanii, halofilin A straight bundles. HalA polymers are highly dynamic and accumulate at positive membrane curvatures in vivo, whereas HalB forms more static foci that localize in areas of local negative curvatures on the outer cell surface. Gene deletions and live- cell imaging show that halofilins are critical in maintaining morphological integrity during shape in accumulation of highly positive curvatures in rods but not in disks. Conversely, disk- shaped cells are exclusively affected by halB deletion, resulting in flatter cells. Furthermore, while Delta halA and Delta halB cells imprecisely determine the future division halofilins might play a significant mechanical scaffolding role in addition to possibly directing envelope synthesis.