FOXD3-mediated transactivation of ALKBH5 promotes neuropathic pain via m6A-dependent stabilization of 5-HT3A mRNA in sensory neurons

• 成果类型: Article
• 署名作者: Huang, Zitong; Zhang, Yuan; Wang, Shoupeng; Qi, Renfei; Sun, Yufang; Tao, Yu; Jiang, Dongsheng; Jiang, Xinghong; Tao, Jin
• 署名单位: Soochow University - China; Soochow University - China; Soochow University - China; Soochow University - China; Helmholtz Association; Helmholtz-Center Munich - German Research Center for Environmental Health; Soochow University - China
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11660
• DOI: 10.1073/pnas.2312861121
• 发表日期: 2024-02-06
• 关键词: spinal nerve ligation; histone acetylation; inflammatory pain; gene-expression; contributes; ondansetron; complex; hdac1

摘要

The N6- methyladenosine (m6A) modification of RNA is an emerging epigenetic regulatory mechanism that has been shown to participate in various pathophysiological processes. However, its involvement in modulating neuropathic pain is still poorly understood. In this study, we elucidate a functional role of the m6A demethylase alkylation repair homolog 5 (ALKBH5) in modulating trigeminal- mediated neuropathic pain. Peripheral nerve injury selectively upregulated the expression level of ALKBH5 in the injured trigeminal ganglion (TG) of rats. Blocking this upregulation in injured TGs alleviated trigeminal neuropathic pain, while mimicking the upregulation of ALKBH5 in intact TG neurons sufficiently induced pain- related behaviors. Mechanistically, histone deacetylase 11 downregulation induced by nerve injury increases histone H3 lysine 27 acetylation (H3K27ac), facilitating the binding of the transcription factor forkhead box protein D3 (FOXD3) to the Alkbh5 promoter and promoting Alkbh5 transcription. The increased ALKBH5 erases m6A sites in Htr3a messenger RNA (mRNA), resulting in an inability of YT521-B homology domain 2 (YTHDF2) to bind to Htr3a mRNA, thus causing an increase in 5-HT3A protein expression and 5-HT3 channel currents. Conversely, blocking the increased expression of ALKBH5 in the injured TG destabilizes nerve injury-induced 5-HT3A upregulation and reverses mechanical allodynia, and the effect can be blocked by 5-HT3A knockdown. Together, FOXD3- mediated transactivation of ALKBH5 promotes neuropathic pain through m6A- dependent stabilization of Htr3a mRNA in TG neurons. This mechanistic understanding may advance the of new for