Pia- FLOW: Deciphering hemodynamic maps of the pial vascular connectome and its to arterial occlusion

• 成果类型: Article
• 署名作者: Gluck, Chaim; Zhou, Quanyu; Droux, Jeanne; Chen, Zhenyue; Glandorf, Lukas; Wegener, Susanne; Razansky, Daniel; Weber, Bruno; El Amki, Mohamad
• 署名单位: University of Zurich; University of Zurich; Swiss Federal Institutes of Technology Domain; ETH Zurich; Swiss Federal Institutes of Technology Domain; ETH Zurich; University of Zurich; University Zurich Hospital; University of Zurich
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11605
• DOI: 10.1073/pnas.2402624121
• 发表日期: 2024-07-09
• 关键词: localization microscopy; blood

摘要

The pial vasculature is the sole source of blood supply to the neocortex. The brain is contained within the skull, a vascularized bone marrow with a unique anatomical connection to the brain meninges. Recent developments in tissue clearing have enabled detailed mapping of the entire pial and calvarial vasculature. However, what are the absolute flow rate values of those vascular networks? This information cannot accurately be retrieved with the commonly used bioimaging methods. Here, we introduce Pia- FLOW, a unique approach based on large- scale transcranial fluorescence localization microscopy, to attain hemodynamic imaging of the whole murine pial and calvarial vasculature at frame rates up to 1,000 Hz and spatial resolution reaching 5.4 mu m. Using Pia- FLOW, we provide detailed maps of flow velocity, direction, and vascular diameters which can serve as ground- truth data for further studies, advancing our understanding of brain fluid dynamics. Furthermore, Pia- FLOW revealed that the pial vascular network functions as one unit for robust allocation of blood after stroke.