Free radical detection in precision- cut mouse liver slices with diamond- based quantum sensing

• 成果类型: Article
• 署名作者: Zhang, Yue; Sigaeva, Alina; Elias-Llumbet, Arturo; Fan, Siyu; Woudstra, Willem; de Boer, Rinse; Escobar, Elkin; Reyes-San-Martin, Claudia; Kisabacak, Robin; Oosterhuis, Dorenda; Gorter, Alan R.; Coenen, Britt; Martinez, Felipe P. Perona; van den Bogaart, Geert; Olinga, Peter; Schirhagl, Romana
• 署名单位: University of Groningen; Universidad de Chile; University of Groningen; Universidad de Antioquia
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11570
• DOI: 10.1073/pnas.2317921121
• 发表日期: 2024-10-22
• 关键词: in-vitro; spin; metabolism; tool; biocompatibility; cytotoxicity; nanodiamond; modulation; incubation; centers

摘要

Free radical generation plays a key role in many biological processes including cell communication, maturation, and aging. In addition, free radical generation is usually elevated in cells under stress as is the case for many different pathological conditions. In liver tissue, cells produce radicals when exposed to toxic substances but also, for instance, in cancer, alcoholic liver disease and liver cirrhosis. However, free radicals are small, short- lived, and occur in low abundance making them challenging to detect and especially to time resolve, leading to a lack of nanoscale information. Recently, our group has demonstrated that diamond- based quantum sensing offers a solution to measure free radical generation in single living cells. The method is based on defects in diamonds, the so- called nitrogen- vacancy centers, which change their optical properties based on their magnetic surrounding. As a result, this technique reveals magnetic resonance signals by optical means offering high sensitivity. However, compared to cells, there are several challenges that we resolved here: Tissues are more fragile, have a higher background fluorescence, have less particle uptake, and do not adhere to microscopy slides. Here, we overcame those challenges and adapted the method to perform measurements in living tissues. More specifically, we used precision- cut liver slices and were able to detect free radical generation during a stress response to ethanol, as well as the reduction in the radical load after adding an antioxidant. Significance Here, we show diamond-based quantum sensing in tissue slices. We demonstrated this in precision cut liver slices which can be maintained alive for several days. This method allows measurements of free radical generation at the nanoscale. We were able to show that we can measure free radical generation during a stress response as well as after adding ascorbic acid.