CXCL13 promotes broad immune responses induced by circular

• 成果类型: Article
• 署名作者: Wan, Jiawu; Wang, Caiqian; Wang, Zongmei; Wang, Lingli; Wang, Haoran; Zhou, Ming; Fu, Zhen F.; Zhao, Ling
• 署名单位: Huazhong Agricultural University; Hubei Hongshan Laboratory; Huazhong Agricultural University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11565
• DOI: 10.1073/pnas.2406434121
• 发表日期: 2024-10-29
• 关键词: follicular helper-cells; messenger-rna vaccine; t-cells; dendritic cells; nanoparticles; chemokines

摘要

Antibody responses induced by current vaccines for influenza and SARS- CoV- 2 often alterations in the immune microenvironment within lymph nodes (LNs) are intricately circular RNA (circRNA) and targeted to LNs, in which CXCL13 was directly integrated into antigen- encoding circRNA strands. We demonstrated that CXCL13 alters the tranCXCL13 promotes the formation of germinal center and elicits robust antigen- specific T cell responses. With the codelivery of CXCL13 and the antigen, CXCL13 enhances cross- reactive antibodies against influenza virus and SARS- CoV- 2, achieving protection against both homologous and heterologous influenza virus challenges in a mouse model. Notably, the targeted modification of LNP surfaces with antibodies helps address some of the challenges associated with lyophilized LNP vaccines, which is crucial for the long- term pression system developed herein provides a simple and robust platform that enhances the magnitude and breadth of antibody responses against multiple viral glycoproteins, highlighting the potential utility of CXCL13 in inducing broad immune responses.