Deletion of TECRL promotes skeletal muscle repair by up- regulating EGR2

• 成果类型: Article
• 署名作者: Geng, Sha; Liu, Song- Bai; He, Wei; Pan, Xiangbin; Sun, Yi; Xue, Ting; Han, Shiyuan; Lou, Jing; Chang, Ying; Zheng, Jiqing; Shi, Xinghong; Li, Yangxin; Song, Yao- Hua
• 署名单位: Soochow University - China; Soochow University - China; Soochow University - China; Suzhou Vocational Health College; Chinese Academy of Medical Sciences - Peking Union Medical College; Fu Wai Hospital - CAMS; Peking Union Medical College
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11377
• DOI: 10.1073/pnas.2317495121
• 发表日期: 2024-05-21
• 关键词: satellite cells; gene-expression; pax7; krox-20; proliferation; regeneration; mechanism; distinct; renewal; GROWTH

摘要

Myogenic regeneration relies on the proliferation and differentiation of satellite cells. TECRL (trans - 2,3 - enoyl - CoA reductase like) is an endoplasmic reticulum protein only expressed in cardiac and skeletal muscle. However, its role in myogenesis remains unknown. We show that TECRL expression is increased in response to injury. Satellite cell - specific deletion of TECRL enhances muscle repair by increasing the expression of EGR2 through the activation of the ERK1/2 signaling pathway, which in turn promotes the expression of PAX7. We further show that TECRL deletion led to the upregulation of the histone acetyltransferase general control nonderepressible 5, which enhances the transcription of EGR2 through acetylation. Importantly, we showed that AAV9 - mediated TECRL silencing improved muscle repair in mice. These findings shed light on myogenic regeneration and muscle repair.