Transient interactions modulate the affinity of NF-KB transcription factors for DNA

• 成果类型: Article
• 署名作者: Li, Tianjie; Shahabi, Shandy; Biswas, Tapan; V. Tsodikov, Oleg; Pan, Wenfei; Huang, De-Bin; Wang, Vivien Ya-Fan; Wang, Yi; Ghosh, Gourisankar
• 署名单位: Chinese University of Hong Kong; University of California System; University of California San Diego; University of Kentucky; University of Macau
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11156
• DOI: 10.1073/pnas.2405555121
• 发表日期: 2024-06-04
• 关键词: kappa-b; c-rel; binding affinity; gene-expression; specificity; DYNAMICS; site; beta; mechanisms; homodimer

摘要

The dimeric nuclear factor kappa B (NF - - K B) transcription factors (TFs) regulate gene expression by binding to a variety of -K B DNA elements with conserved G:C - rich flanking sequences enclosing a degenerate central region. Toward defining mechanistic principles of affinity regulated by degeneracy, we observed an unusual dependence of the affinity of RelA on the identity of the central base pair, which appears to be noncontacted in the complex crystal structures. The affinity of -K B sites with A or T at the central position is - 10 - fold higher than with G or C. The crystal structures of neither the complexes nor the free -K B DNAs could explain the differences in affinity. Interestingly, differential dynamics of several residues were revealed in molecular dynamics simulation studies, where simulation replicates totaling 148 mu s were performed on NF --K B:DNA complexes and free - K B DNAs. Notably, Arg187 and Arg124 exhibited selectivity in transient interactions that orchestrated a complex interplay among several DNA - interacting residues in the central region. Binding and simulation studies with mutants supported these observations of transient interactions dictating specificity. In combination with published reports, this work provides insights into the nuanced mechanisms governing the discriminatory binding of NF --K B family TFs to - K B DNA elements and sheds light on cancer pathogenesis of cRel, a close homolog of RelA.