Antigenic cartography using variant- specific hamster sera reveals substantial antigenic variation among Omicron subvariants

• 成果类型: Article
• 署名作者: Muehlemann, Barbara; Trimpert, Jakob; Walper, Felix; Schmidt, Marie L.; Jansen, Jenny; Schroeder, Simon; Jeworowski, Lara M.; Beheim-Schwarzbach, Jorn; Bleicker, Tobias; Niemeyer, Daniela; Richter, Anja; Adler, Julia M.; Vidal, Ricardo Martin; Langner, Christine; Vladimirova, Daria; Wilks, Samuel H.; Smith, Derek J.; Voss, Mathias; Paltzow, Lea; Christophersen, Christina Martinez; Rose, Ruben; Krumbholz, Andi; Jones, Terry C.; Corman, Victor M.; Drosten, Christian
• 署名单位: Free University of Berlin; Humboldt University of Berlin; Charite Universitatsmedizin Berlin; Free University of Berlin; Humboldt University of Berlin; Humboldt University of Berlin; Free University of Berlin; Charite Universitatsmedizin Berlin; Berlin Institute of Health; German Center for Infection Research; Free University of Berlin; University of Cambridge; University of Kiel; University of Kiel; Schleswig Holstein University Hospital; Labor Berlin - Charite Vivantes GmbH
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11136
• DOI: 10.1073/pnas.2310917121
• 发表日期: 2024-08-06
• 关键词: antibody evasion; sars-cov-2; infection; neutralization; vaccination

摘要

Severe acute respiratory syndrome Coronavirus 2 (SARS-- CoV-2)- 2) has developed substantial antigenic variability. As the majority of the population now has pre-- existing immunity due to infection or vaccination, the use of experimentally generated animal immune sera can be valuable for measuring antigenic differences between virus variants. Here, we immunized Syrian hamsters by two successive infections with one of nine SARS-CoV-2- CoV- 2 variants. Their sera were titrated against 16 SARS-CoV-2- CoV- 2 variants , the resulting titers were visualized using antigenic cartography. The antigenic map shows a condensed cluster containing all pre-- Omicron variants (D614G, Alpha, Delta, Beta, Mu, , an engineered B.1+E484K variant) and considerably more diversity among a selected panel of Omicron subvariants (BA.1, BA.2, BA.4/BA.5, the BA.5 descendants BF.7 and BQ.1.18, the BA.2.75 descendant BN.1.3.1, the BA.2-- derived recombinants XBB.2 and EG.5.1, and the BA.2.86 descendant JN.1). Some Omicron subvariants were as antigenically distinct from each other as the wildtype is from the Omicron BA.1 variant. Compared to titers measured in human sera, titers in hamster sera are of higher magnitude, show less fold change, and result in a more compact antigenic map topology. The results highlight the potential of sera from hamsters for the continued antigenic characterization of SARS-- CoV-2.- 2.