Microbial community interactions on a chip

• 成果类型: Article
• 署名作者: Juang, Duane S.; Wightman, Wren E.; Lozano, Gabriel L.; Juang, Terry D.; Barkal, Layla J.; Yu, Jiaquan; Garavito, Manuel F.; Hurley, Amanda; Venturelli, Ophelia S.; Handelsman, Jo; Beebe, David J.
• 署名单位: University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison; University of Wisconsin System; University of Wisconsin Madison; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Boston Children's Hospital
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11121
• DOI: 10.1073/pnas.2403510121
• 发表日期: 2024-09-24
• 关键词: bacillus-cereus uw85; gut microbiota; susceptibility; nodulation; diversity; diffusion; GROWTH; field

摘要

Multispecies microbial communities drive most ecosystems on Earth. Chemical and biological interactions within these communities can affect the survival of individual members and the entire community. However, the prohibitively high number of possible interactions within a microbial community has made the characterization of factors that influence community development challenging. Here, we report a Microbial Community Interaction (mu CI) device to advance the systematic study of chemical and biological interactions within a microbial community. The mu CI creates a combinatorial landscape made up of an array of triangular wells interconnected with circular wells, which each contains either a different chemical or microbial strain, generating chemical gradients protein provided the target readout in the triangular wells, and antibiotics or microorthat gentamicin and vancomycin are antagonistic to each other in inhibiting the target B. cereus UW85, displaying weaker inhibitory activity when used in combination than alone. We identified three- member communities constructed with isolates from the plant both strain- level and community- level insight. The scalable geometric design of the influence microorganisms in solitary or community life.