Shade- induced ROS/NO reinforce COPT- mediated diffuse cell growth

• 成果类型: Article
• 署名作者: Iglesias, Maria Jose; Rojas, Cecilia Costigliolo; Bianchimano, Luciana; Legris, Martina; Schon, Jonas; Grozeff, Gustavo Esteban Gergoff; Bartoli, Carlos Guillermo; Blazquez, Miguel A.; Alabadi, David; Zurbriggen, Matias D.; Casal, Jorge J.
• 署名单位: Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); Leloir Institute; University of Buenos Aires; University of Buenos Aires; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); Consejo Superior de Investigaciones Cientificas (CSIC); Universitat Politecnica de Valencia; CSIC-UPV - Instituto de Biologia Molecular y Celular de Plantas (IBMCP); Heinrich Heine University Dusseldorf; National University of La Plata; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); National University of La Plata; Museo La Plata; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); University of Buenos Aires
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-11116
• DOI: 10.1073/pnas.2320187121
• 发表日期: 2024-10-15
• 关键词: nitric-oxide; hypocotyl growth; auxin; light; responses; gene; photomorphogenesis; loop; identification; phytochromes

摘要

Canopy shade enhances the activity of PHYTOCHROME INTERACTING FACTORs (PIFs) to boost auxin synthesis in the cotyledons. Auxin, together with local PIFs and promotes hypocotyl growth to facilitate access to light. Whether shade alters the cellular redox status thereby affecting growth responses, remains unexplored. Here, we show that, under shade, high auxin levels increased reactive oxygen species and nitric oxide accumulation in the hypocotyl of Arabidopsis. This nitroxidative environment favored the promotion of hypocotyl growth by COP1 under shade. We demonstrate that COP1 is S- nitrosylated, particularly under shade. Impairing this redox regulation enhanced COP1 degradation by the proteasome and diminished the capacity of COP1 to interact with target proteins and to promote hypocotyl growth. Disabling this regulation also generated transversal asymmetries in hypocotyl growth, indicating poor coordination among different cells, which resulted in random hypocotyl bending and predictably low ability to compete with neighbors. These findings highlight the significance of redox signaling in the control of diffuse growth during shade avoidance.