Clonal barcoding of endogenous adult hematopoietic stem cells reveals a spectrum of lineage contributions

• 成果类型: Article
• 署名作者: Feng, Jue; Jang, Geunhyo; Esteva, Eduardo; Adams, Nicholas M.; Jin, Hua; Reizis, Boris
• 署名单位: New York University; Icahn School of Medicine at Mount Sinai; New York University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-10735
• DOI: 10.1073/pnas.2317929121
• 发表日期: 2024-01-23
• 关键词: progenitors

摘要

The hierarchical model of hematopoiesis posits that self- renewing, multipotent hemato-poietic stem cells (HSCs) give rise to all blood cell lineages. While this model accounts for hematopoiesis in transplant settings, its applicability to steady- state hematopoiesis remains to be clarified. Here, we used inducible clonal DNA barcoding of endogenous adult HSCs to trace their contribution to major hematopoietic cell lineages in unma-nipulated animals. While the majority of barcodes were unique to a single lineage, we also observed frequent barcode sharing between multiple lineages, specifically between lymphocytes and myeloid cells. These results suggest that both single- lineage and multi-lineage contributions by HSCs collectively drive continuous hematopoiesis, and highlight a close relationship of myeloid and lymphoid development