An organic O donor for biological hydroxylation reactions

• 成果类型: Article
• 署名作者: Ferizhendi, Katayoun Kazemzadeh; Simon, Philippe; Pelosi, Ludovic; Sechet, Emmanuel; Arulanandam, Roache; Chehade, Mahmoud Hajj; Rey, Martial; Onal, Deniz; Flandrin, Laura; Chreim, Rouba; Faivre, Bruno; Vo, Samuel Chau-Duy-Tam; Arias-Cartin, Rodrigo; Barras, Frederic; Fontecave, Marc; Bouveret, Emmanuelle; Lombard, Murielle; Pierrel, Fabien
• 署名单位: VetAgro Sup; Communaute Universite Grenoble Alpes; Institut National Polytechnique de Grenoble; Universite Grenoble Alpes (UGA); Centre National de la Recherche Scientifique (CNRS); CNRS - Institute for Engineering & Systems Sciences (INSIS); Universite PSL; College de France; Sorbonne Universite; Pasteur Network; Universite Paris Cite; Institut Pasteur Paris; Pasteur Network; Universite Paris Cite; Institut Pasteur Paris; Centre National de la Recherche Scientifique (CNRS)
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-10702
• DOI: 10.1073/pnas.2321242121
• 发表日期: 2024-03-20
• 关键词: escherichia-coli; oxygen activation; biosynthesis; PATHWAY; prephenate; ubiquinone; arogenate; ACID; gene

摘要

All biological hydroxylation reactions are thought to derive the oxygen atom from one of three inorganic oxygen donors, O2, H2O2, or H2O. Here, we have identified the organic compound prephenate as the oxygen donor for the three hydroxylation steps of the O2 - independent biosynthetic pathway of ubiquinone, a widely distributed lipid coenzyme. Prephenate is an intermediate in the aromatic amino acid pathway and genetic experiments showed that it is essential for ubiquinone biosynthesis in Escherichia coli under anaerobic conditions. Metabolic labeling experiments with 18O-shikimate, a precursor of prephenate, demonstrated the incorporation of 18O atoms into ubiquinone. The role of specific iron-sulfur enzymes belonging to the widespread U32 protein family is discussed. Prephenate- dependent hydroxylation reactions represent a unique biochemical strategy for adaptation to anaerobic environments.