PARP1 UFMylation ensures the stability of stalled replication forks

• 成果类型: Article
• 署名作者: Gong, Yamin; Wang, Zhifeng; Zong, Wen; Shi, Ruifeng; Sun, Wenli; Wang, Sijia; Peng, Bin; Takeda, Shunichi; Wang, Zhao-Qi; Xu, Xingzhi
• 署名单位: Shenzhen University; Leibniz Association; Leibniz Institut fur Alternsforschung - Fritz-Lipmann-Institut (FLI); Shandong University; Friedrich Schiller University of Jena
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-10478
• DOI: 10.1073/pnas.2322520121
• 发表日期: 2024-04-30
• 关键词: dna; brca2; poly(adp-ribosyl)ation; activation; restart; stress; xrcc1

摘要

The S - phase checkpoint involving CHK1 is essential for fork stability in response to fork stalling. PARP1 acts as a sensor of replication stress and is required for CHK1 activation. However, it is unclear how the activity of PARP1 is regulated. Here, we found that UFMylation is required for the efficient activation of CHK1 by UFMylating PARP1 at K548 during replication stress. Inactivation of UFL1, the E3 enzyme essential for UFMylation, delayed CHK1 activation and inhibits nascent DNA degradation during replication blockage as seen in PARP1 - deficient cells. An in vitro study indicated that PARP1 is UFMylated at K548, which enhances its catalytic activity. Correspondingly, a PARP1 UFMylation - deficient mutant (K548R) and pathogenic mutant (F553L) compromised CHK1 activation, the restart of stalled replication forks following replication blockage, and chromosome stability. Defective PARP1 UFMylation also resulted in excessive nascent DNA degradation at stalled replication forks. Finally, we observed that PARP1 UFMylation - deficient knock - in mice exhibited increased sensitivity to replication stress caused by anticancer treatments. Thus, we demonstrate that PARP1 UFMylation promotes CHK1 activation and replication fork stability during replication stress, thus safeguarding genome integrity.