Origin and evolution of auxin- mediated acid growth

• 成果类型: Article
• 署名作者: Zeng, Hai Yue; Deng, Shiyu; Jin, Congcong; Shang, Zhiyun; Chang, Le; Wang, Jiajun; Han, Xue; Wang, Ao; Jin, Dan; Wang, Yubo; He, Hang; Li, Lanxin; Deng, Xing Wang; Wei, Ning
• 署名单位: Southwest University - China; Peking University; Peking University; Peking University; Peking University; China Agricultural University; Southwest University - China
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-10398
• DOI: 10.1073/pnas.2412493121
• 发表日期: 2024-12-17
• 关键词: membrane h+-atpase; cell-wall extension; plasma-membrane; binding protein-1; pectate lyase; elongation; phosphorylation; phosphatases; transport; ethylene

摘要

The classical acid growth theory suggests that auxin stimulates cell expansion by triggering apoplast acidification via plasma membrane (PM)- localized H+- ATPase. Here, we reconstructed the origin and evolutionary history of auxin- mediated acid growth. Comparative phylogenomic analysis showed that most core components of acid growth originated in Charophyta and then underwent subclass expansion and functional innovation during plant terrestrialization. In Charophyceae algae Chara braunii, we found that PM H+- ATPase has formed a core regulatory module with TMK and PP2C.D, which can be activated by photosynthesis- dependent phosphorylation through light rather than auxin. Despite the lack of canonical auxin receptor TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F- BOX (TIR1/AFB), auxin elicits significant internodal elongation and transcriptional reprogramming in C. braunii, implying the existence of an ancient auxin- mediated growth mechanism. We propose that the evolution of acid growth represents a neofunctional adaptation to terrestrial environments, in which PM H+- ATPase in carbon concentrating for photosynthesis was utilized to acidify apoplast for cell expansion, and the core components responsible for acid growth eventually established a regulatory network in land plants by connecting with the TIR1/ AFB pathway.