Myosin XI- mediated BIK1 recruitment to nanodomains facilitates FLS2-BIK1 complex formation during innate immunity in Arabidopsis

• 成果类型: Article
• 署名作者: Wang, Bingxiao; Zhou, Zhaoyang; Zhou, Jian-Min; Li, Jiejie
• 署名单位: Beijing Normal University; China Agricultural University; Chinese Academy of Sciences; Institute of Genetics & Developmental Biology, CAS; Yazhouwan National Laboratory; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Beijing Normal University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-10250
• DOI: 10.1073/pnas.2312415121
• 发表日期: 2024-06-18
• 关键词: f-actin organization; nadph oxidase rbohd; membrane nanodomains; receptor complex; plasma-membrane; kinase bik1; plant; microdomains; interacts; TRAFFICKING

摘要

Plants rely on immune receptor complexes at the cell surface to perceive microbial molecules and transduce these signals into the cell to regulate immunity. Various immune receptors and associated proteins are often dynamically distributed in specific nanodomains on the plasma membrane (PM). However, the exact molecular mechanism and functional relevance of this nanodomain targeting in plant immunity regulation remain largely unknown. By utilizing high spatiotemporal resolution imaging and single - particle tracking analysis, we show that myosin XIK interacts with remorin to recruit and stabilize PM - associated kinase BOTRYTIS - INDUCED KINASE 1 (BIK1) within immune receptor FLAGELLIN SENSING 2 (FLS2) - containing nanodomains. This recruitment facilitates FLS2/BIK1 complex formation, leading to the full activation of BIK1 - dependent defense responses upon ligand perception. Collectively, our findings provide compelling evidence that myosin XI functions as a molecular scaffold to enable a spatially confined complex assembly within nanodomains. This ensures the presence of a sufficient quantity of preformed immune receptor complex for efficient signaling transduction from the cell surface.