The RPD3L deacetylation complex is required for facultative heterochromatin repression in Neurospora crassa

• 成果类型: Article
• 署名作者: Mumford, Colleen C.; Tanizawa, Hideki; Wiles, Elizabeth T.; McNaught, Kevin J.; Jamieson, Kirsty; Tsukamoto, Kenta; Selker, Eric U.
• 署名单位: University of Oregon; Hokkaido University; University of California System; University of California Irvine; United States Department of Energy (DOE); Oak Ridge National Laboratory; Reed College - Portland
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-10222
• DOI: 10.1073/pnas.2404770121
• 发表日期: 2024-08-06
• 关键词: histone deacetylase; transcriptional repression; integral component; dna methylation; gene; RECRUITMENT; ume6; acetylation; corepressor; antagonizes

摘要

Repression of facultative heterochromatin is essential for developmental processes in repressive complex 2 is a prominent feature of facultative heterochromatin in both fungi and higher eukaryotes. Although this methylation is frequently associated with silencing, the detailed mechanism of repression remains incompletely understood. We utilized a forward genetics approach to identify genes required to maintain silencing at facultative heterochromatin genes in Neurospora crassa and identified three previously uncharacterized genes that are important for silencing: sds3 (NCU01599), rlp1 (RPD3L protein 1; RLP2 associate with N. crassa homologs of the Saccharomyces cerevisiae Rpd3L complex and are required for repression of a subset of H3K27- methylated genes. Deletion of these genes does not lead to loss of H3K27 methylation but increases acetylation of histone H3 lysine 14 at up- regulated genes, suggesting that RPD3L- driven deacetylation is a in other organisms.