Overlapping role of synaptophysin and synaptogyrin family proteins in determining the small size of synaptic vesicles
成果类型：
Article
署名作者：
Park, Daehun; Fujise, Kenshiro; Wu, Yumei; Lujan, Rafael; Del Olmo-Cabrera, Sergio; Wesseling, John F.; De Camilli, Pietro
署名单位：
Yale University; Yale University; Yale University; Catholic University of Korea; Catholic University of Korea; Universidad de Castilla-La Mancha; Universidad Miguel Hernandez de Elche
刊物名称：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN/ISSBN：
0027-9999
DOI：
10.1073/pnas.2409605121
发表日期：
2024-07-16
关键词：
摘要：
Members of the synaptophysin and synaptogyrin family are vesicle proteins with four transmembrane domains. In spite of their abundance in synaptic vesicle (SV) membranes, their role remains elusive and only mild defects at the cellular and organismal level are observed in mice lacking one or more family members. Here, we show that coexpression with synapsin in fibroblasts of each of the four brain-- enriched members of this family-synaptophysin, synaptoporin, synaptogyrin 1, and synaptogyrin 3-is sufficient to generate clusters of small vesicles in the same size range of SVs. Moreover, mice lacking all these four proteins have larger SVs. We conclude that synaptophysin and synaptogyrin family proteins play an overlapping function in the biogenesis of SVs and in determining their small size.