Transition of signal requirement in hematopoietic stem cell development from hemogenic endothelial cells

• 成果类型: Article
• 署名作者: Morino-Koga, Saori; Tsuruda, Mariko; Zhao, Xueyu; Oshiro, Shogo; Yokomizo, Tomomasa; Yamane, Mariko; Tanigawa, Shunsuke; Miike, Koichiro; Usuki, Shingo; Yasunaga, Kei - ichiro; Nishinakamura, Ryuichi; Suda, Toshio; Ogawa, Minetaro
• 署名单位: Kumamoto University; Kumamoto University; Tokyo Women's Medical University; Kumamoto University; Institute of Science Tokyo; Tokyo Medical & Dental University (TMDU); RIKEN; Kumamoto University; Kumamoto University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9995
• DOI: 10.1073/pnas.2404193121
• 发表日期: 2024-07-30
• 关键词: yolk-sac; thrombopoietin; maturation; establishment; progenitor; receptor; origin; hscs

摘要

in vivo during mouse embryogenesis. When cultured in vitro, cells from the embryo phenotypically defined as pre-HSC-I and pre-HSC-II have the potential to differentiate into HSCs. However, minimal factors required for HSC induction from HECs have not yet been determined. In this study, we demonstrated that stem cell factor 11.5 pre-HSC-I in a serum-free and feeder-free culture condition. In contrast, E10.5 pre-HSC-I and HECs required an endothelial cell layer in addition to SCF and TPO to differentiate into HSCs. A single-cell RNA sequencing analysis of E10.5 to 11.5 dorsal aortae with surrounding tissues and fetal livers detected TPO expression confined in hepatoblasts, while SCF was expressed in various tissues, including endothelial cells and hepatoblasts. Our results suggest a transition of signal requirement during HSC a beneficial tool for exploring the molecular mechanisms underlying the development of HSCs from HECs.