Four decades of Eukaryotic DNA replication: From yeast genetics to high- resolution cryo- EM structures of the replisome

• 成果类型: Article
• 署名作者: Tye, Bik-Kwoon
• 署名单位: Cornell University
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9971
• DOI: 10.1073/pnas.2415231121
• 发表日期: 2024-10-15
• 关键词: origin recognition complex; rna-polymerase-ii; mcm proteins; cmg helicase; minichromosome maintenance; ribosomal-subunit; s-phase; binding; initiation; transcription

摘要

I had my eyes set on DNA replication research when I took my first molecular biology course in graduate school. My election to the National Academy of Sciences came just when I was retiring from active research. It gives me an opportunity to reflect on my personal journey in eukaryotic DNA replication research, which started as a thought experiment and culminated in witnessing the determination of the cryoelectron microscopic structure of the yeast replisome in the act of transferring histone- encoded epigenetic information at the replication fork. I would like to dedicate this inaugural article to my talented trainees and valuable collaborators in gratitude for the joy they gave me in this journey. I also want to thank my mentors who instilled in me the purpose of science. I hope junior scientists will not be disheartened by the marathon nature of research, but mindful enough to integrate and pause for other equally fun and meaningful activities of life into the marathon.