Fgf10 mutant newts regenerate normal hindlimbs despite severe developmental defects

• 成果类型: Article
• 署名作者: Suzuki, Miyuki; Okumura, Akinori; Chihara, Akane; Shibata, Yuki; Endo, Tetsuya; Teramoto, Machiko; Agata, Kiyokazu; Bronner, Marianne E.; Suzuki, Ken- ichi T.
• 署名单位: California Institute of Technology; National Institutes of Natural Sciences (NINS) - Japan; National Institute for Basic Biology (NIBB); Aichi Gakuin University; National Institutes of Natural Sciences (NINS) - Japan; National Institute for Basic Biology (NIBB)
• 刊物名称: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• ISSN/ISSBN: 0027-9826
• DOI: 10.1073/pnas.2314911121
• 发表日期: 2024-03-12
• 关键词: limb regeneration; expression; redundant; pattern; genes; fgf-8

摘要

In amniote limbs, Fibroblast Growth Factor 10 (FGF10) is essential for limb development, but whether this function is broadly conserved in tetrapods and/or involved in adult limb regeneration remains unknown. To tackle this question, we established Fgf10 mutant lines in the newt Pleurodeles waltlwhich has amazing regenerative ability. While Fgf10 mutant forelimbs develop normally, the hindlimbs fail to develop and downregulate FGF target genes. Despite these developmental defects, Fgf10 mutants were able to regenerate normal hindlimbs rather than recapitulating the embryonic phenotype. Together, our results demonstrate an important role for FGF10 in hindlimb formation, but little or no function in regeneration, suggesting that different mechanisms operate during limb regeneration versus development.